| 학회 | 한국공업화학회 |
| 학술대회 | 2015년 봄 (04/29 ~ 05/01, BEXCO (부산)) |
| 권호 | 19권 1호 |
| 발표분야 | (생물공학) 생물전환기술 현황과 전망 |
| 제목 | Multistep enzymatic synthesis of medium chain carboxylic acids from long chain fatty acids: from biosynthetic pathway design to process development |
| 초록 | C9 to C13 ω-hydroxycarboxylic acids, ω-aminocarboxylic acids, and α,ω-dicarboxylic acids, which are widely used for the production of various chemical products and intermediates, can be produced from renewable oils and fatty acids (e.g., soybean oil, microalgae oil, oleic acid, linoleic acid, ricinoleic acid) 1-4. ω-Hydroxycarboxylic acids, which were produced from oxidative cleavage of fatty acids via enzymatic reactions involving fatty acid double bond hydratases, alcohol dehydrogenases, Baeyer-Villiger monooxygenases and esterases, were further converted into ω-aminocarboxylic acids or α,ω-dicarboxylic acids by the AlkJ from Pseudomonas putida GPo1 and ω-transaminase from Silicibacter pomeroyi 1,2. The recombinant Escherichia coli expressing the catalytic enzymes and the whole-cell based biocatalytic process were constructed to produce a variety of C9 to C12 carboxylic acids including 1,9-nonanedioic acid, 1,10-dec-6-enedioic acid, 11-hydroxyundec-9-enoic acid, 11-aminoundecanoic acid, and 12-hydroxydodec-6,9-dienoic acid from ricinoleic acid, oleic acid, linoleic acid or γ-linolenic acid to high yield 1-5. This study thus contributes to the preparation of functional carboxylic acids from renewable unsaturated fatty acids. |
| 저자 | 박진병 |
| 소속 | 이화여자대 |
| 키워드 | multistep enzymatic synthesis; medium chain carboxylic acids; fatty acids; biosynthetic pathway design; process development |
