| 학회 |
한국재료학회 |
| 학술대회 |
2017년 봄 (05/17 ~ 05/19, 목포 현대호텔) |
| 권호 |
23권 1호 |
| 발표분야 |
B. 나노화학/바이오 분과 |
| 제목 |
Label-free biosensor using optical properties of organic nanostructures |
| 초록 |
We reported on the protein sensing using a light-emitting nano structures (NSs). The aptamer were easily attached to the light-emitting NSs through electrostatic interaction between the negative counter-ions and the terminal amine (NH3+) group attached at the end of the aptamer. After the functionalization aptamer and their label–free recognition of target protein onto the surface of light-emitting NSs, the light-emitting color and intensity of a light-emitting NS were dramatically changed due to the conformational changes of the protein main chains and fluorescence resonance energy transfer. We observed color change of a light-emitting NS after attaching the aptamer, and then luminescence intensity of a single light-emitting NS was dramatically enhanced by hybridizing protein. The conformational changes of the light-emitting NS main chains due to attaching aptamer were investigated ultraviolet-visible absorption and confocal Raman spectra. The enhanced PL of the aptamer/protein can be explained in terms of the dopant-mediated fluorescence chain reaction. |
| 저자 |
이호진, 김석호, 최진호, 김형태, 김도형, 박동혁
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| 소속 |
인하대 |
| 키워드 |
<P>label free; biosensor; optical property; aptamer; luminescence; raman scattering</P>
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| E-Mail |
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