| 초록 |
Alginate/bacterial cellulose (BC) nanocomposite beads, with well-controlled size and regular spherical shapes, were simply prepared via the entrapment of Gluconacetobacter xylinus into barium alginate hydrogel beads, followed by the cultivation of the entrapped cells in culture media with low sodium ion concentration. The entire surface of the alginate hydrogel beads containing cells were covered with cellulose fibers after 36 h of cultivation. The cellulose crystallinity index of the alginate/BC beads was 0.7. The water vapor sorption capacity of the alginate/BC beads increased significantly from 0.07 to 38.00 (g/g dry bead) as cultivation time increased. The immobilization of lipase on the surface of the nanocomposite beads was also investigated. The activity and specific activity of lipase immobilized on alginate/BC beads was 2.6 and 3.8 times higher, respectively, than that of lipase immobilized on cellulose beads. The alginate/BC nanocomposite beads prepared in this study have several potential applications in biocatalytic, biomedical, and pharmaceutical fields due to their biocompatibility, biodegradability, high crystallinity, and large surface area. |
