| 초록 |
In this study, dexamethasone was conjugated to PAMAM dendrimer (generation 0) and its gene transfection efficiency was investigated. Dexamethasone is the potent ligand of the glucocorticoid receptor which facilitates the transfer into nucleus, and it is known to enlarge the nuclear pore complexes. The PAMAM-dexamethasone conjugate (PAM-Dex) was synthesized by a one-step reaction method using Traut’s reagent. The product was further purified using HPLC to obtain a single dexamethasone-conjugated derivative. The purified product was confirmed by MALDI-TOF MS and 1H NMR. To prepare a liposomal structure, DOPE was used as a helper lipid. In vitro transfection assay showed that the PAM-Dex/ DOPE liposome/DNA complex displayed higher gene delivery efficiency compared to PAMAM/DNA complex. In addition, PAM-Dex/ DOPE liposomes showed the lowest toxicity compared to PAMAM, PEI 25kD and Lipofectamine. |
