| 초록 |
A cyclodextrin (CD) polyrotaxane (PR) structure was synthesized as a targeted and stimuli responsive drug delivery carrier. A DNA aptamer (DNAa), i-motif DNA (DNAi) and its complimentary DNA (cDNAi) were each conjugated to αCD. These 3 types of CD-DNAs formed a pseudo-PR (pPR) with diaminopolyethylene glycol. Here, one pPR contained CD-DNAi and CD-DNAa, while another had CD-cDNAi and CD-DNAa. Finally, both ends of pPR were capped with bulky molecule to yield two different PRs. By annealing them, a DNA crosslinked nanoconstruct had been formed. An anticancer agent Doxorubicin was intercalated between the double-stranded DNA and released by the formation of i-motif structure in endosomal pH. Targeting ability was introduced by cancer targeting aptamer. With easily replaceable targeting moiety by modular design and controllable drug loading capacity by altering feed ratio, this system will enable patient-specific treatment with active targeting ability in a stimuli-responsive manner. |
