| 학회 | 한국화학공학회 |
| 학술대회 | 2006년 봄 (04/20 ~ 04/21, 대구 인터불고 호텔) |
| 권호 | 12권 1호, p.412 |
| 발표분야 | 생물화공 |
| 제목 | Development of Shuttle Vectors for Succinic Acid Producing Bacteria |
| 초록 | In order to obtain improved succinic acid producing bacteria by metabolic engineering, shuttle vectors for Mannheimia succiniciproducens, Actinobacillus succinogenes and Escherichia coli were constructed. The pMVD, pME and pMEx vectors were manipulated fusing a Mannheimia varigena plasmid pMVSCS1 with an E. coli expression vector pKK223-3. The performance of the vectors was evaluated by cloning a fumC gene into M. succiniciproducens and A. succinogenes. The stability of the vectors was also tested by using ampicillin as a selection marker. The quantitative real-time PCR method was used to analyze the plasmid copy number. The increased level of the activity and expression of the fumarase encoded by the fumC gene in the recombinant M. succiniciproducens and A. succinogenes suggests that the vectors constructed in this work can be used for engineering them as a succinic acid producer. [This work was supported by the Genome-based Integrated Bioprocess Project of the Ministry of Science and Technology. Further supports by the LG Chem Chair Professorship, IBM SUR program, Brain Korea 21 project, and by the KOSEF through the Center for Ultramicrochemical Process Systems are appreciated.] |
| 저자 | 이정욱, 장유신, 송효학, 이상준, 이상엽 |
| 소속 | 한국과학기술원 |
| 키워드 | Shuttle vector; Succinic acid; Mannheimia succiniciproducens |
| 원문파일 | 초록 보기 |
