| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 봄 (05/01 ~ 05/03, 부산 벡스코(BEXCO)) |
| 권호 |
23권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Artificial lactam riboswitch for high-throughput screening and selection |
| 초록 |
Caprolactam is a monomer used for the synthesis of nylon-6. An intracellular biosensor for caprolactam can facilitate high-throughput metabolic engineering of recombinant microbial strains. Because of the mixed production of caprolactam and valerolactam in the recombinant strain, a caprolactam biosensor should be highly specific for caprolactam. However, a highly specific caprolactam sensor has not been reported. Here, we developed an artificial riboswitch that specifically responds to caprolactam. This riboswitch was prepared using a coupled in vitro-in vivo selection strategy with a heterogeneous pool of RNA aptamers obtained from in vitro selection to construct a riboswitch library used in in vivo selection. The caprolactam riboswitch successfully discriminated caprolactam from valerolactam. This riboswitch enabled caprolactam-dependent control of cell growth, which will be useful for improving caprolactam production and is a valuable tool for metabolic engineering. |
| 저자 |
장성연1, 장성호1, 임대균2, 강택진3, 오민규2, 정규열1
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| 소속 |
1포항공과대, 2고려대, 3동국대 |
| 키워드 |
Caprolactam; riboswitch; SELEX; Metabolite biosensor; high-throughput screening
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| E-Mail |
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