| 초록 |
RNA splicing plays important roles in transcriptome and proteome diversity. Herein, we describe the use of nanoplasmonic system that unveils RNA folding and assembly during pre-mRNA splicing. With a couple of SERS-probes and plasmonic probes binding at the boundary sites of exon-2/intron-2 and intron-2/exon-3 of β-globin gene’s pre-mature RNA, the splicing process brings the probes in the plasmonic bands. For plasmonic probes, a plasmon shift increase of 29 nm is measured by plasmonic coupling. The increased scattering intensity and SERS fingerprinting reveal a clear dynamics of pre-mRNA splicing. Moreover, a time-resolved experiment of individual RNA molecules exhibit a successful splicing and inhibited splicing event by 33 μM biflavonoid isoginkgetin, inhibitor of RNA splicing. The results suggest that the RNA splicing is successfully monitored on the nanoplasmonic system. Thus, this platform can be useful for studying RNA nanotechnology and biomolecular folding. |
