| 학회 | 한국공업화학회 |
| 학술대회 | 2016년 가을 (10/26 ~ 10/28, 제주국제컨벤션센터(ICCJEJU)) |
| 권호 | 20권 2호 |
| 발표분야 | 생체재료_포스터 |
| 제목 | Phylogeny-guided Mining of Soil Metagenomes for the Discovery of New Biologically Active Aromatic Polyketides |
| 초록 | DNA sequence is increasingly being used to guide the discovery of biosynthetic gene clusters capable of encoding for novel natural products. When studying cultured bacteria, it is possible to carry out detailed analyses of complete biosynthetic gene clusters. However, this is not possible when exploring complex metagenomes because of their immense sizes. To circumvent this limitation, we have used highly-conserved natural product biosynthetic genes as phylogenetic markers for guiding the discovery of functionally novel natural product biosynthetic gene clusters from metagenome. In this approach, biosynthetic genes are PCR-amplified, and the resulting amplicon sequences are aligned with sequences from functionally characterized gene clusters to identify sequences that encode for novel secondary metabolties. Here, I demonstrate the use of a single gene phylogeny-based mining approach to guide the discovery of new aromatic polyketides from achieved metagenomic libraries. |
| 저자 | 강학수 |
| 소속 | 건국대 |
| 키워드 | metagenomics; natural products; polyketides |
