| 학회 |
한국화학공학회 |
| 학술대회 |
2010년 봄 (04/22 ~ 04/23, 대구 EXCO) |
| 권호 |
16권 1호, p.359 |
| 발표분야 |
생물화공 |
| 제목 |
SERS-active Nano structure for biomolecule detection |
| 초록 |
We here present an optofluidic surface enhanced Raman spectroscopy (SERS) device for on-chip detection of vasopressin using an aptamer based binding assay.To create the SERS-active substrate, densely packed, 200 nm diameter, metal nanotube arrays were fabricated using an anodized alumina nanoporous membrane as a template for shadow evaporation.We explore the use of both single layer Au structures and multilayer Au/Ag/Au structures and also demonstrate a facile technique for integrating the membranes with all PDMS microfluidic devices.Using the integrated device, we demonstrate a linear response in the main detection peak intensity to solution phase concentration and a limit of detection on the order of 50 ng/mL.This low limit of detection is obtained with device containing the multilayer SERS substrate which we show exhibits a stronger Raman enhancement while maintaining biocompatibility and ease or surface reactivity with the capture probe. |
| 저자 |
허윤석1, 정아람2, 김해진3, David Erickson4
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| 소속 |
1Division of Material Science, 2Korea Basic Science Institute, 3Sibley School of Mechanical and Aerospace Engineering, 4Cornell Univ. |
| 키워드 |
SERS; AAO
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| E-Mail |
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| 원문파일 |
초록 보기 |
