| 학회 |
한국화학공학회 |
| 학술대회 |
2010년 가을 (10/21 ~ 10/22, 대전컨벤션센터) |
| 권호 |
16권 2호, p.1595 |
| 발표분야 |
생물화공 |
| 제목 |
On-Chip Concentration of Bacteria via Cluster Formation by Capillary Electrophoresis |
| 초록 |
An on-chip concentration of bacteria via a “cluster” formation by applying an electric field is described. Food-borne pathogenic bacterium cells spontaneously formed clusters, approximately 28 μm in diameter, in the microchannel and continuously concentrated at the edge of the microchannel by capillary electrophoresis (CE). In this study, while 3% PEO or 20% polyvinylpyrrolidone (PVP) solution placed in the anode reservoir, the bacterium cells in the cathode reservoir migrated and formed clusters in the microchannel, 100 μm in inner diameter and 10 mm in length, after applying an electric field. Moreover, the formed clusters of Listeria monocytogenes were continuously stacked at the edge of the microchannel. The formed cluster size is more than 15-fold larger than the size of a food-borne bacterium cell, which is approximately 2 μm in length and 0.5 μm in diameter. Both of the cluster formation and aggregation of the clusters enhance the concentration efficiency. |
| 저자 |
김영호1, Amanda Neer2, 김동주3, 김연상4
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| 소속 |
1경북대, 2Department of Mechanical Engineering, 3Auburn Univ., 4United States |
| 키워드 |
Biochip; Capillary electrophoresis; Bacteria
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| E-Mail |
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| 원문파일 |
초록 보기 |
