| 초록 |
In recent years, two-photon microscopy (TPM), which employs two lower energy, near-infrared photons for excitation, has emerged as vital tool for live-tissue imaging owing to the advantages of increased penetration depth, localized excitation, and prolonged observation time. Thus, TPM allows molecular imaging deep inside intact tissues for a long period of time. However, there is a need to develop targeted and efficient two-photon probes that can make TPM a real vital tool in biological and medicinal studies. In this seminar, I will describe small molecular two-photon probes that can detect the intracellular free metal ions, thiols, nitric oxide, and hydrogen peroxide in live cells and living tissues. |
