| 초록 |
Here, we designed a series of polymeric bilayers composed of poly(glycidyl methacrylate) (pGMA) and poly(2-(dimethylamino)ethyl methacrylate) (pDMAEMA), designated pGMA:pDMAEMA, using initiated chemical vapor deposition (iCVD). Harnessing the surface-growing characteristics of iCVD polymer films, we were able to precisely engraft acetylcholine-like functionalities (tertiary amine and quaternary ammonium) onto cell culture plates. Notably, pGD3, a pGMA:pDMAEMA preparation with the highest surface composition of quaternary ammonium, fostered the most rapid outgrowth of neural cells. Moreover, brain-derived neurotrophic factor (BDNF) level continuously accumulated in pGD3-cultured neurons, reaching a 3-fold increase at 50 DIV. Taken together, our findings indicate that a polymer thin film with optimal acetylcholine-like functionality enables unprecedented long-term culture and survival of primary neurons. |
