| 학회 |
한국공업화학회 |
| 학술대회 |
2015년 봄 (04/29 ~ 05/01, BEXCO (부산)) |
| 권호 |
19권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Activation of pentose phosphate pathway in Escherichia coli for the co-production of hydrogen and ethanol from glucose |
| 초록 |
To address low H2 production yield by dark fermentation, co-production of H2 and ethanol by E. coli from glucose, was suggested in this study. Moreover, ethanol is one of the attractive biofuel producible through the fermentation. In order to increase the cofactor availability for ethanol production, operation of Pentose-Phosphate (PP) pathway was tried by deleting pgi in the base strain (E. coli BW25113 ΔhycA ΔhyaAB ΔhybBC ΔldhA ΔfrdAB). Additional over-expression of G6PHD and 6PGDH recovered the cell growth of Δpgi mutant as well as increased co-production yield by decreasing acetate formation. Resulting strain increased co-production yield (1.65 for H2 and 1.38 mol/mol for ethanol) which corresponds to ~85 % energy recovery from glucose showing the possibility of operating PP pathway under anaerobic condition. |
| 저자 |
설은희, SUNDARA SEKAR BALAJI, Satish Kumar Ainala, 박성훈
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| 소속 |
부산대 |
| 키워드 |
Hydrogen production; Escherichia coli; co-production
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| E-Mail |
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