| 초록 |
With the increase in demand for biodegradable polyester composed of 1,3 propanediol (1,3-PD), the microbial production of 1,3-PD has received much attention. However, till now, native microorganisms directly producing 1,3-PD from glucose was not identified and/or studied. Most microorganisms prefer glucose and glucose takes most portion in lignocellulosic biomass composed of various carbohydrates. Although using glucose is beneficial, there are only few reports on the production of 1,3-PD from glucose. In this study, we focus on development of strains that can convert glucose to 1,3-PD. For these purpose, two genes encoding glycerol-3-phosphate dehydrogenase and glycerol-3-phosphatase from Saccharomyces cerevisiae were overexpressed. Further, the produced glycerol was converted to 1,3-PD using native enzymes in Klebsiella pneumoniae J2B. Efficient recombinant strains developed in our study could lead to economical production of 1,3-PD. |
