| 학회 |
한국공업화학회 |
| 학술대회 |
2016년 봄 (05/02 ~ 05/04, 여수 엑스포 컨벤션) |
| 권호 |
20권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Co-production of 3-hydroxypropionic acid and 1,3-propanediol using glycerol by Klebsiella pneumoniae J2B: Reduction of by-products accumulation |
| 초록 |
Microbial 3-HP production from glycerol requires glycerol dehydratase (GDHt) and aldehyde dehydrogenase (ALDH). GDHt requires coenzyme B12 and ALDH requires NAD+ as cofactors for the production of 3-HP from glycerol. The imbalance between GDHt and ALDH activities cause the accumulation of toxic intermediate, 3-hydroxypropionaldehyde (3-HPA). As a solution to these problems, co-production of 1,3-PDO along with 3-HP has been suggested. 1,3-PDO production could regenerate NAD+ and utilize 3-HPA, thus avoiding 3-HPA accumulation and also facilitate the process to be performed under microaerobic conditions. But acetate was produced as major metabolite, which is very toxic to the cells. Therefore, in this study, we attempted the down-regulation of glycolytic pathway and disruption of byproducts formation pathway. We also attempted to operate TCA cycle/glyoxylate shunt to reduce the carbon traffic at pyruvate node. |
| 저자 |
고연주, 설은희, 권성진, 이재현, 발라지, 박성훈
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| 소속 |
부산대 |
| 키워드 |
3-HP; 1; 3-PDO; Klebsiella pneumoniae
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| E-Mail |
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