| 초록 |
In this work, we investigated kinetic resolution for preparation of (S)-styrene oxide using the epoxide hydrolase of Mugil cephalus which was protein-engineered based on comparative homology modeling. We used the freeze-dried recombinant Escherichia coli as the whole-cell catalyst. We optimized reaction conditions such as temperature, substrate concentration, addition of surfactants and reaction time for an efficient enantioselective hydrolysis of racemic styrene oxide at high concentration. We carried out batch kinetic resolution of 3.0 M racemic styrene oxide in the presence of 25% (v/v) tween 20 and 2.5% (v/v) glycerol at 10oC. As a result, we prepared (S)-styrene oxide with 99% ee and 45% yield after 8 h reaction using the recombinant E. coli as the biocatalyst. |
