| 초록 |
The industrially useful substances 3-hydroxypropionic acid and 1,3-propanediol are producible by glycerol. It is possible to selectively increase the each metabolites, 3-HP or 1,3-PDO by regulation of intracellular NADH/NAD+ ratio. Bioelectrochemical system enables to regulate intracellular redox state (NADH/NAD+) using electron trasnfer. In this study, we attempted to actively control the production of 3-HP and 1,3-PDO via bioelectrochemical control using Escherichia coli. For this active control, heterologous gene expression was designed which are regulated by NADH/NAD+-bind regulator, Sox, Rex. As a regult, the synthesis of 3-HP was enhanced by lowering the intracellular NADH/NAD+ ratio when the oxidation potential was given. When the reduction potential was given, the ratio of NADH/NAD+ was increased to induce the synthesis of 1,3-PDO whereas 3-HP synthesis can be suppressed. Thus, it makes us to know the real-time redox changes, and to produce substances selectively. |
