| 초록 |
Secreted Antigen is one of the proteins expressed in Rosellinia necatrix causing White Root Rot. White Root Rot occurs in fruit trees such as apples, peaches, and pear trees, and it shrinks fruit size and reduces fruit yield. Therefore, information on the Secreted Antigen may be helpful in understanding the White Root Rot. In this study, the Secreted Antigen gene was amplified by PCR and purified using a kit. This purified product was ligated into pET21a vector and this recombinant plasmid was transformed into competent cell through heat shock. BL21 (DE3) pLysS, LEMO (DE3) and Rosetta-gami (DE3) were used as competent cells. Transformed cells were cultured varying temperature and IPTG concentration, and expressed proteins were identified by SDS-PAGE. This work was carried out with the support of "Cooperative Research Program for Agriculture Science & Technology Development (Project title: Development of diagnostic method for major tree fruit diseases and Biological control, Project No: PJ01191701)" Rural Development Administration, Republic of Korea. |
