| 학회 | 한국고분자학회 |
| 학술대회 | 2004년 가을 (10/08 ~ 10/09, 경북대학교) |
| 권호 | 29권 2호, p.53 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Elastic Biodegradable Polymers and Mechano-active Tissue engineering |
| 초록 | Tissue engineering has a purpose of replacing lost or malfunctioning tissues with new healthy tissues by using cell and scaffold. To engineer good organ and tissues, it is important to the signals that cell receive from their micro-environments including cell binding molecules, cell-cell adhesion interactions, soluble growth factors, mechanical stimuli and so on. Many recent studies have reported that mechanical stimuli enhanced the development and function of engineered tissues. So it is likely to be necessary the development of scaffolds which can maintain their mechanical integrity and delivers the mechanical signals to adherent cells during mechanical strain application. In other words, to generate healthy tissues from cell, the scaffolds must be elastic and capable of withstanding cyclic mechanical strain without cracking or significant permanent deformation. Therefore, it is very important to synthesize elastic biodegradable polymers and fabricate them into scaffold. Aliphatic polyester such as polyglycolide (PGA), polylactide (PLA), poly(-caprolactone) (PCL) have received great interest in tissue engineering application. We synthesized elastic biodegradable polymers by combining these components. Copolymer having different molecular structure such as random, tri-block or multi-block showed different elastic properties. poly(glycolide-co-caprolactone) (PGCL) and poly(lactide-co-caprolactone) (PLCL) copolymers were studied intensively. We also investigated degradation properties as well as mechanical properties of these copolymers. Elastic biodegradable polymers were fabricated into sheet or tubular scaffolds by extrusion / particulate leaching method. The PLCL scaffold of porosity 90% was very flexible to have a elongation up to 200 % but rubber-like elastic to exhibit a completely recovery for 1 week and to withstand for 2 weeks under cyclic stress (10 % strain, 1Hz frequency) in culture media at 37 oC. We studied small-diameter vascular grafts engineered with pulsatile mechanical strains using elastic, biodegradable 3D tubular scaffolds. Tubular scaffolds were fabricated with synthesized poly(lactide-co-caprolactone) (PLCL) by extrusion/particulate leaching, and the resulting scaffolds were very soft and elastic. VSMCs (vascular smooth muscle cells) and ECs (enthothelial cells) were cultured on the scaffolds in vitro under a pulsatile flow on a bioreactor. We hypothesized that mechanical stimuli induces the feature of engineered tissues to be similar to that of VSMCs and ECs in native vascular tissues. We also studied cartilage tissue engineering with cyclic compressive mechanical strains using elastic, biodegradable 3D scaffolds. Acknowledgement: This work was supported by the Korea Ministry of Science and Technology, M6-0302-00-0017 REFERENCES 1. A. J. Banes, "Mechanical Strain and the Mammalian cell" in: Physical force and the Mammalian cell, J. A. Frangos, ed., Academic press, San Diego, pp81-123 (1993). 2. L. E. Niklason, J. Gao, W. M. Abbott, K. K. Hirschi, S. Houser, R. Marini and R. Langer, Science, 284, 489 (1999). 3. S. P. Hoerstrup, G. Zund, R. Sodian, A. M. Schnell, J. Grunenfelder, M. I. Turina, European J of Cardio-thoracic Surgery, 20, 164-169, (2001). 4. B.-S. Kim and D. J. Mooney, J. Biomech. Eng. 122, 210 (2000). |
| 저자 | 김수현, 김영하 |
| 소속 | 한국과학기술(연) |
| 키워드 | 분해성고분자; 조직공학; 탄성고분자; Mechano-active |
