| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 봄 (04/14 ~ 04/15, 전경련회관) |
| 권호 | 30권 1호, p.649 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Direct measurement of molecular interaction between proteins and model cell membrane |
| 초록 | The importance of innate immunity in recognizing microbial pathogens and the response against them is now widely recognized. Especially CD14 and LPS binding protein (LBP) are known to play important roles in the pathway leading to the endotoxic shock caused by LPS of Gram negative bacteria cell membrane.1 The kinetic behavior of these protein-CD14 , LBP- has been investigated and found that they have a high affinity to LPS.2 However the adhesion force between CD14 , LBP and LPS has never been investigated. So we will measure the adhesion force between CD14,LBP and LPS by changing the loading rate using Atomic Force Microscopy(AFM) , by changing the structure of LPS using LPS mutant (Ra,Rc,Rd1,Rd2,Re). AFM tip will be modified by special surface synthesis for decreasing nonspecific binding. LPS will be immobilized to the model biomembrane including lipid bilayer for more similar natural membrane. Kinetic parameters obtained from the relation between the adhesion force and loading rate could be used to thermodynamically characterize the binding behavior of these molecules. Additionally, as many reports show, the directly measured force will be used to explore the energy landscape of ligand-receptor unbinding.3 Fig.1. F-D curve of LPS Ra-CD14 1. Gallay, P., Heumann, D., Le Roy, D., Barras, C. and Glauser, Proc. Natl. Acad. Sci. USA, 91, 7922 (1994) . 2. Celestine J. Thomasa, Mili Kapoora, Shilpi Sharmaa, Huguette Bausingerb, Umit Zyilanb,Dan Lipskerb, Daniel Hanaub, and Avadhesha Suroliaa ,FEBS letters, 531,184 (2002) 3. Merkel, R.Nassoy, P.Leumg, A.Ritchie, and K.Evans, Nature,397,50 (1999) |
| 저자 | 김종수, 장순남, 김의숙, 김성수, 조길원 |
| 소속 | 포항공대 |
| 키워드 | lipid bilayer; force measure; bio-interface; dynamic force spectroscopy |
