| 학회 |
한국화학공학회 |
| 학술대회 |
1999년 가을 (10/22 ~ 10/23, 경남대학교) |
| 권호 |
5권 2호, p.3177 |
| 발표분야 |
생물화공 |
| 제목 |
재조합 곤충세포에서 생산된 인간 인터루킨-2의 녹색형광단백질과 금속친화배위자와의 융합을 통한 분리정제 |
| 초록 |
Fusion protein of green fluorescent protein and human interleukin-2 was produced in insect Trichoplusia ni larvae infected with recombinant baculovirus derived from the Autographa californica nuclear polyhedrosis virus (AcNPV). The additional histidine residues on fusion protein enabled the efficient purification of fusion protein based on IMAC. Addition to advantages of GFP as a fusion marker, GFP was able to be used as a selectable purification marker; we easily determined the correct purified fusion protein sample fraction by simply detecting GFP fluorescence.
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| 저자 |
차형준, William E. Bentley
|
| 소속 |
포항공과대 |
| 키워드 |
Purification; Insect Cells; Human Intereulin-2; Green Fluorescent Protein; Metal Affinity Ligand
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| E-Mail |
, |
| 원문파일 |
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