| 초록 |
Delivery of small interference RNA (siRNA) allows for the inhibition of gene expression in tumor cells. Different from other chemical drugs, it has low cytotoxicity when delivered to human body and effectively knock-down gene expression of tumor cells. Here we describe a novel enzymatically synthetic method to produce DNA-RNA hybrid nanoparticles (DRNPs) to effectively deliver siRNA to target site. DRNPs entirely comprise of DNA-based aptamer and siRNAs and thus are non-toxic and biocompatible. In addition, DNA-RNA hybridization and G-quartet-forming oligonucleotides contained within DRNPs have resistance of nuclease degradation, therefore could overcome some limitations associated with nucleic acid-based therapeutics in vivo. |
