| 초록 |
An siRNA has recognized as a potential drug due to its target-specific gene silencing. However, due to their innate physiochemical characteristics, the systemic delivery of naked siRNA fails to achieve therapeutically relevant gene silencing. To this end, a variety of cationic polymers and lipids have extensively studied for efficient siRNA delivery. In this study, we demonstrate the structure and function relation of nucleic acid nanoparticles for siRNA delivery. To achieve target-specific delivery, translationally controlled tumor protein-protein transduction domain (TCTP-PTD) conjugated siRNA was linked to either holiday or tetrahedron nanostructures via self-assembly, forming siRNA-TCTP-PTD(TCTP)/DNA nanoparticles. Two distinct shapes, holiday junction and tetrahedron, has been tested for the delivery of siRNA-TCTP conjugate and siRNA-TCTP-holiday conjugates and their in vitro gene silencing efficacy is evaluated in detail. |
