| 학회 |
한국고분자학회 |
| 학술대회 |
2006년 봄 (04/06 ~ 04/07, 일산킨텍스) |
| 권호 |
31권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Gene delivery system for targeting the artery wall using low molecular water-soluble chitosan |
| 초록 |
Gene therapy is a method for the treatment of diseases with introducing the gene-engineered materials into a patient with gene-deficiency disease or cancer to produce a therapeutic protein in a patient’s cells. The various gene carriers, chitosan, a natural cationic polysaccharide, is continued to attract a great deal of interest as a candidate non-viral vector for gene delivery because of its low cytotoxicity and high positive charges. Targeted gene carrier was synthesized to target artery wall cells using LMWSC. AWBP-PEG-g-LMWSC was synthesized by conjugaion the artery wall binding peptide (AWBP) to the end of PEGylation LMWSC as a gene carrier to target artery wall cells. Characters of the synthesized AWBP-PEG-g-LMWSC were analyzed by measuring FT-IR, 1H-NMR, zeta-potentiometer and atomic force microscopy (AFM). The formation of plasmid/LMWSC complex was proved and transfection efficiency in 293T cells was evaluated. The cytotoxicity of LMWSC also was determined by MTT assay. |
| 저자 |
장민자1, 장미경1, 김동곤1, 박준규1, 장학수1, 권중근2, 서동혁1, 나재운1
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| 소속 |
1순천대, 2조선이공대 |
| 키워드 |
chitosan. gene delivery system; artery wall;
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| E-Mail |
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