| 초록 |
Fibrin formation, which is caused by the interaction between fibrinogen and thrombin, was controlled by varying the conditions and the change of viscoelastic property was investigated by quartz crystal microbalance with dissipation monitoring (QCM-D). Here, QCM-D was shown as a useful tool for simultaneous measurements of the mass change and the viscoelastic property of fibrin formation. Depending on the concentration or injection order of thrombin, antithrombin and heparin, the viscoelastic property of fibrin layer was clearly different. In the absence of heparin, preabsorbed rigid fibrinogen layer converted into more viscous fibrin layer after addition of thrombin. When heparin and antithrombin were added before thrombin injection, the storage and loss modulus of fibrin changed a lot depending on the concentrations of thrombin, antithrombin, and heparin. AFM was used to characterize the final morphology of polymerized fibrin layers and supported the QCM-D results. |
