| 초록 |
In the past several decades, there have been significant advances in understanding of protein sequences, structures and functions which in turn have aided design and engineering of proteins with novel structures and/or functions. In this talk, I would like to introduce our current research activities toward engineering synthetic proteins. The recruitment of new amino acid constituents provides the protein engineering field with new tools and raises the prospects for creating novel proteins. Even though several approaches have been developed, each one, however, has suffered from limitations such as a low expression yield or scarification of one amino acid for unnatural amino acid. In the first part of this talk, I will present a new method to incorporate amino acid analogues into proteins which could overcome the issues of current methods. Autoinhibition is nature’s mechanism of regulating protein activity. Autoinhibited proteins have inhibitory regions which can be displaced through conformational changes or removed by cleavage events. In the second part, I will describe a new protease assay method based on an engineered autoinhibited protein and enzyme-linked immunoassay. |
