| 초록 |
Engineering protease with desired specificity and activity has a great interest in understanding proteases as well as developing new therapeutics. In this talk, I will describe a new reporter, autoinhibited protein, for protease activity and demonstrate the feasibility of this method by finding OmpT endopeptidase variants that cleave AR sequence instead of its original target sequence, RR. In addition, I will introduce our effort to develop sensitive protease sensors using the autoinhibited protein; the abnormal activity of proteases is observed in many clinical diseases. |
