| 초록 |
Addition of amino acid analogues into the set of building blocks for protein synthesis has expanded the scope of protein engineering. The two approaches, amber suppression and codon reassignment, have been developed and optimized for incorporating unnatural amino acids into recombinant proteins in vivo. However, each method has suffered from limitations such as the efficiency in incorporation into multiple sites or the site specificity, even though some modifications have been tried to overcome them. In this presentation, we will introduce a new strategy to incorporate amino acid analogues into protein via rare codons, which will provide a powerful tool for engineering proteins with novel compositions. |
