| 초록 |
To enhance the deliveryof therapeutic genes in to the cells, the ideal gene carriersare still under investigation. Cationic liposomesare useful tools for gene delivery, but most of the them are subjected various obstacles, unexpected binding to blood component, immune reaction and toxic effects. In this study, we reported stabilized plasmid-lipid particles (SPLPs) with enzymatically cleavable oligopeptides. This uniform structure of SPLP can overcome limitations cationic liposome system. Also, SPLP with enzymatically cleavable oligopeptide for ability to improve transfection efficiency. The GFLG sequence is cleaved by the enzyme cathepsin-B and it may promote endosomal escape after intracellular internalization. We purified uncapsulated DNA using DEAE-sepharose chromatography and measured diameter with DLS. The encapsulated DNA was analyzed through lysisby TritonX-100 and PicoGreen assay. Transfection efficiency and cytotoxicity were determined with luciferase and MTT bromide assays. |
