| 학회 |
한국고분자학회 |
| 학술대회 |
2008년 봄 (04/10 ~ 04/11, 컨벤션 뷰로(대전)) |
| 권호 |
33권 1호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
Effect of rat BMSC by co-culture system with PLGA/DBP scaffolds seeded OEC and SC |
| 초록 |
We confirmed that effects of PLGA/DBP scaffolds seeded OEC and SC on proliferation and differentiation of BMSCs. We designed control groups that BMSCs cultured with only PLGA and PLGA/DBP scaffold, and experimental groups that BMSCs cultured with PLGA/DBP scaffold seeded OECs, SCs or OECs and SCs(OECs/SCs). BMSCs viability was determined by using MTT assays. RT-PCR was used to BDNF, NSE and S-100 in BMSCs. In MTT assays result, cell proliferation in PLGA/DBP scaffolds seeded OECs, SCs, and OECs/SCs, respectively was increased during culture period. BDNF, NSE and S-100 gene expressions were weakly detected in BMSCs cultured with PLGA/DBP scaffold seeded only one type cells, and were strongly expressed in the group of PLGA/DBP scaffold seeded OECs and SCs. This phenomenon was expected that the growth factor or cytokine in DBP and molecules secreted by OECs and SCs affect to proliferation and differentiation of BMSCs. Acknowledgements This research was supported by SCRC (SC3100). |
| 저자 |
김수미1, 김순희1, 김초민1, 오아영1, 김근아1, 이종문1, 이해방2, 강길선1
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| 소속 |
1전북대, 2한국화학(연) |
| 키워드 |
DBP; PLGA; OEC; SC; BMSC
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| E-Mail |
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