| 초록 |
Utilization of cheap carbon sources are both beneficial to production cost and economic feasibility. In the previous study, production of itaconate from acetate was constructed with rational metabolic engineering strategy, proving the potential of acetate as a promising carbon source. In this study, we introduced combinatorial approach to further increase the production of itaconate. Initially, the itaconate-specific screening device could be constructed using transcriptional regulator and selection marker. Thus, Adaptive Laboratory Evolution (ALE), utilizing natural selection toward microorganisms under selection pressure, was introduced to itaconate-producing strain. The evolved strain could produce 1.06 g/L of itaconate, a 1.24-fold increase in comparison with parental strain and the yield was significantly enhanced by 7.16-fold. The rational approach will be applied to maximize the itaconate production and the mutant will be additionally anaylzed with WGS for validation. |
