| 학회 |
한국공업화학회 |
| 학술대회 |
2016년 가을 (10/26 ~ 10/28, 제주국제컨벤션센터(ICCJEJU)) |
| 권호 |
20권 2호 |
| 발표분야 |
생체재료_포스터 |
| 제목 |
Self-assembled Y-RNA structure of CRISPR/CAS9 for a multifunctional genome editing |
| 초록 |
The RNA-guided nuclease CRISPR (clustered regularly interspaced short palindromic repeat)/CRISPR-associated (Cas) protein 9 system is a new emerging gene editing approach in the field of gene therapy. In this study, we prepared the self-assembled Y-RNA nanostructures as multifunctional guide RNAs (gRNA) for Cas9 delivery. To prepare each arm of Y-RNA structures, we utilized the rolling circle amplification of DNA template. Amplified RNA products were treated with RNase H and the length of gRNA was carefully controlled by site-specific cleavage. After complexation of Cas9 with gRNA, three distinct Cas9/gRNA complexes can self-assemble to form Y- RNA structures for region-specific (loco-specific) gene editing in mammalian cells. Efficient and programmable gene editing has been demonstrated in MEF cells, which co-express the green (G), red (R), and blue (B) fluorescence proteins. |
| 저자 |
김민정, 김보영, 권효경, 정한샘, 이규리, 이혁진
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| 소속 |
이화여자대 |
| 키워드 |
CRISPR/Cas9; gene editing
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| E-Mail |
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