| 초록 |
we show that self-assembled functional nucleic nanostructures with a well-defined size and structure can serve as a multi-functional platform to induce efficient RNA interference, mRNA expression, and CRISPR/Cas9 mediated gene editing. For the synthesis of functional RNA structures, we have utilized the rolling circle transcription of pre-designed DNA template to produce a large quantity of functional RNAs. For programmable and simultaneous gene silencing, we have design the dicer substrate RNA nanostructures to regulate three different fluorescent proteins in the target cells. For mRNA expression, in vitro transcription of mRNA has been carried out to express three distinct fluorescent proteins in target cells. Long term expression can be obtained with the programmable assembly of mRNA into defined RNA structures. |
