| 초록 |
Gene therapeutics for infectious diseases introduce a target-specific treatment strategy which can overcome problems of current broad spectrum antibiotics. In this study, we apply the CRISPR system, a pioneering tool for efficient genome editing, to genetically modulate molecular targets in bacterial infections via nonviral delivery. Recombinant Cas9 was derivatized with a cationic polymer and packaged with single guide RNA targeting the infection. We showed that polymer derivatized CRISPR complexes (= Cr-Nanocomplex) were successfully formed while maintaining endonuclease function. We also demonstrate that the Cr-Nanocomplex can be efficiently delivered into both bacteria and mammalian cells. Furthermore, the Cr-Nanocomplex showed higher genome editing efficiency compared to using native or lipid-based formulations. The present study shows a therapeutic strategy which can be potentially applied in the clinic for treating infections as well as various other diseases. |
