| 학회 | 한국화학공학회 |
| 학술대회 | 2019년 가을 (10/23 ~ 10/25, 대전컨벤션센터) |
| 권호 | 25권 2호, p.1507 |
| 발표분야 | 생물화공 (Biochemical Engineering) |
| 제목 | Parallel Droplet Generation with Linear Concentration Gradient |
| 초록 | Conventional methods for generating concentration gradient have employed serial 2-fold dilution strategy. It is a simple way to generate the chemical concentration gradients in a single device. However, it has a low linearity on their gradient profiles as well as the limitation to generate non-linear gradient due to its dilution system. Moreover, improvement of linearity requires complicate design to precisely control the fluidic resistance, making the device to have low space efficiency because of its huge size. We present a novel microfluidic platform producing monodisperse droplets with linear concentration gradient. It is achieved by simply tuning the flow resistance of two aqueous phases in each parallel droplet generator. We verified the linearity of the gradient by visualizing it with fluorescence. The results well-matched with the theroetical value which is obatained through calculating the flow resistance. We further demonstrated the controllability of the gradient profile by showing various types of slopes including ‘V’ and ‘N’-shaped concentration profiles. We believe that our strategy has a great potential to be used in many bio/chemical assays. |
| 저자 | 김재성1, 이병진1, 정헌호2, 이창수1 |
| 소속 | 1충남대, 2전남대 |
| 키워드 | 생물화학공학 |
| 원문파일 | 초록 보기 |
