| 학회 |
한국공업화학회 |
| 학술대회 |
2018년 봄 (05/02 ~ 05/04, 대구 엑스코(EXCO)) |
| 권호 |
22권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Efficient 5-aminolevulinic acid production through elaborate flux redistribution toward glyoxylate shunt in Escherichia coli |
| 초록 |
Microbial production of 5-aminolevulinic acid (ALA) has received much attention because of its potential in clinical applications. Overexpression of related enzymes and an transporter yielded remarkable achievements in ALA production. Nonetheless, there is significant room for carbon flux optimization to enhance ALA production. The aim of this study was precise carbon flux optimization for high ALA production in Escherichia coli. Initially, genes hemA and hemL were overexpressed with strong promoters and synthetic 5'-untranslated regions (5'-UTRs). Then, TCA cycle was blocked to force carbon flux toward the ALA production pathway by deletion of sucA. In spite of metabolic imbalance in the strain, further precise tuning of carbon flux to the glyoxylate cycle by varying the transcriptional strength of aceA led to substantially improved cell growth and ALA production. |
| 저자 |
예대열1, 노명현1, 임현규1, 박성훈2, 서상우3, 정규열1
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| 소속 |
1포항공과대, 2울산과학기술원, 3서울대 |
| 키워드 |
5-aminolevulinic acid; glyoxylate shunt; flux redistribution
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| E-Mail |
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