| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 봄 (05/10 ~ 05/12, 광주 김대중컨벤센센터(Kimdaejung Convention Center)) |
| 권호 |
21권 1호 |
| 발표분야 |
생물공학 |
| 제목 |
Paper-based neuraminidase assay sensor for detection of influenza viruses |
| 초록 |
In this study, we described a paper-based neuraminidase assay sensor (PNAS) that can be applied to detect infections caused by influenza viruses. The PNAS was designed and manufactured to quantitatively identify the levels of neuraminidase in the sample, which is based on colorimetric analysis using the X-Neu5Ac substrate. We have demonstrated that Michaelis-Menten kinetics applies to PNAS. The kinetic studies indicate that PNAS provides a good condition for the enzyme reaction (Km = 8.327 × 10-3 M), which is close to the value calculated from the enzyme reaction rate calculation in solution (Km = 0.89 × 10-3 M). In addition, since PNAS is a paper-based sensor, it is very easy to dispose of it after use. In conclusion, the PNAS developed from this study is expected to be applicable to the rapid and safe detection of influenza viruses. |
| 저자 |
황철환1, 박한규1, 송원석2, 안다희1, 정윤이1, 김성민1, 김윤곤1
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| 소속 |
1숭실대, 2서울대 |
| 키워드 |
Influenza virus; Neuraminidase; Paper chip; Enzyme kinetics; X-Neu5Ac; Colorimetric assay
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| E-Mail |
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