| 학회 |
한국화학공학회 |
| 학술대회 |
2010년 봄 (04/22 ~ 04/23, 대구 EXCO) |
| 권호 |
16권 1호, p.371 |
| 발표분야 |
생물화공 |
| 제목 |
Production of Polylactic Acid and its Copolymers by Metabolically Engineered Escherichia coli |
| 초록 |
Polylactic acid (PLA) is a promising biomass-derived polymer, but is synthesized by a two-step process: fermentative production of lactic acid followed by chemical polymerization. Here we report production of PLA homopolymer and its copolymer, poly(3-hydroxybutyrate-co-lactate), by direct fermentation of metabolically engineered E. coli. Introduction of the heterologous metabolic pathways involving engineered propionate CoA-transferase and polyhydroxyalkanoate synthase allowed synthesis of PLA and P(3HB-co-LA) in E. coli. The metabolic pathways of E. coli were further engineered based on in silico genome-scale metabolic flux analysis. PLA homopolymer and P(3HB-co-LA) copolymers containing up to 70 mol% lactate could be produced up to 11 wt% and 46 wt% from glucose, respectively.This work was supported by LG Chem and by the Korean Systems Biology Research Project (20090065571) of the Ministry of Education, Science and Technology (MEST) through the National Research Foundation of Korea (NRF). Further supports by the World Class University Program (R32-2008-000-10142-0) of the MEST, LG Chem Chair Professorship, IBM SUR program, and Microsoft are appreciated. |
| 저자 |
최용준1, 정유경1, 김태용1, 박시재2, 이상엽1
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| 소속 |
1KAIST, 2KRICT |
| 키워드 |
Polylactic acid; homopolymer; propionate CoA-transferase; polyhydroxyalkanoate synthase
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| E-Mail |
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| 원문파일 |
초록 보기 |
