| 초록 |
The three-dimensional (3D) scaffold play an important role in tissue engineering. Particularly, interconnected pore of 3D scaffold affects not only nutrient delivery but also cell proliferation and ECM formation. The 3D bioprinting is a useful technology for fabricating the 3D scaffold with desired pore size. In this study, we used gelatin as a bioink and fabricated pore size controlled 3D scaffolds by low-temperature printing system. The produced 3D scaffold was crosslinked by EDC/NHS. For confirmation of 3D scaffold surface morphologies and porosity, the scanning electron microscopy was used. The crosslinked 3D scaffold pore sizes were approximately 30 % reduced than that of a designed. Biological properties of 3D scaffold were evaluated by MTT assay. After 14 days of human dermal fibroblast culture, the optical density of 3D scaffold with pore size more than 580 μm was approximately 14% higher than that on the 3D scaffold with 435 μm of pore size. |
