| 학회 |
한국고분자학회 |
| 학술대회 |
2007년 봄 (04/12 ~ 04/13, 제주 ICC) |
| 권호 |
32권 1호 |
| 발표분야 |
나노바이오기술용 고분자(의료용 고분자 부문위원회) |
| 제목 |
The cellular physiological behavior on defined patterns of extracellular matrix |
| 초록 |
Cell adhesion is an importance step for cell shape, cell survival, and proliferation for anchorage-dependant cells, whose dimension can be controlled by micro-or nano patterning of cell adhesive extracellular matrix. We fabricated polydimethylsiloxane stamp using the replica molding by E-beam lithography. Micropatterned fibronectin of 4 μm width strip was spaced from 2 μm to 20 μm, was confirmed by immunofluorescence staining. Fibroblasts were attached only on the fibronectin–coated micropattern at 4 h after plating. The cell alignment at the gap smaller than 10 μm was random but that at the gap larger than 10 μm was longitudinal along the fibronectin micropatterns. Therefore our fibronectin micropatterning suggests that filopodia protrusion may be limited by the adhesive gap larger than 10 μm and the confinement of cell dimension on the longitudinal and narrowly surface also negatively affect cell proliferation even though sufficient surface area are provided longitudinally. |
| 저자 |
Chun Ho Kim |
| 소속 |
Korea Institute of Radiological and Medical Sciences |
| 키워드 |
|
| E-Mail |
|
