| 학회 |
한국고분자학회 |
| 학술대회 |
2014년 가을 (10/06 ~ 10/08, 제주 ICC) |
| 권호 |
39권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Bioreducible Polymer and Graphene Oxide for Enhancing Chondrogenic Differentiation and Therapeutic Angiogenesis of Adult Stem Cells |
| 초록 |
Graphene oxide (GO) can be utilized as both a cell-adhesion substrate and a growth factor protein-delivery carrier for the chondrogenic differentiation of human adult stem cells (hASCs). Conventionally, chondrogenic differentiation of stem cells is achieved by culturing cells in pellets and adding the TGF-β3 to the culture medium. We utilized GO sheets to adsorb fibronectin (FN) and TGF-β3 and were then incorporated in pellets of hASCs. The hybrid pellets of hASC-GO enhanced the chondrogenic differentiation of hASCs by adding the cell-FN interaction and supplying TGF-β3. We also developed a branched poly(disulfide amine) (B-PDA) for intracellular delivery of Fas siRNA into hASCs. The Fas-silenced hASCs can then be formulated as enlarged stem cell spheroids and transplanted into mouse ischemic hind limbs as paracrine secretors for therapeutic angiogenesis. These methods may provide a new platform for stem cell application for regenerative medicine. |
| 저자 |
방석호, 김병수
|
| 소속 |
서울대 |
| 키워드 |
Angiogenesis; Bioreducible Polymer; Graphene oxide; Chondrogenic Differentiation
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| E-Mail |
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