| 초록 |
Amphiphilic diblock copolymers were self-assembled to form a novel structure, polymersome. Polymersome is a vesicle that is formed from diblock or triblock copolymers. Although its structure is similar to that of liposome, polymersome has wider range of size and has greatly enhanced stability as well as it is easier to be modified with ligands and stealth property. In this study, we prepared various polymersomes with various classes of mPEG-PLA(methoxy poly(ethylene glycol)-poly(lactic acid)) synthesized by ring-opening polymerization. On the first hand, we showed polymersome formation by conjugating Rhodamine on hydroxyl end groups of mPEG-PLA as well as by solubilizing hydrophobic dye, Nile Red, in the hydrophobic phase. Size control and stability were tested by varying total molecular weight, volume fraction and conformation of mPEG-PLA. It was shown that volume fraction was the primary controlling factor. Various polymersome preparation methods as liposome methods and self-assembly methods were tested. It was shown that self-assembly methods formed polymersomes with greater stability as observed with optical microscope and confocal microscope. |
