초록 |
Energy production using biocatalysts has been studied and reported. The process of production of 2,3-Butanediol using carbon monoxide as feed stock has both market ability and commercial viability. However, 2,3-BDO is very hydrophilic chemical so it takes a lot of time and cost to purify it. It is necessary to convert 2,3-BDO to other hydrophobic products directly in medium. A recombinant Pseudomonas putida KT2440 can uptake 2,3-BDO and produce mevalonate. Mevalonate is an intermediate in the pathway for production of terpenoid. In order to show that 2,3-BDO is more suitable carbon source for mevalonate production than other carbon sources, experiments about cell growth and mevalonate production were performed with various carbon sources such as 2,3-BDO, glucose, and glycerol. The amount of produced mevalonate from 2,3-BDO was 1.90 g/L, and those of glucose and glycerol were 0.11 g/L and 0.18 g/L, respectively, for 48 hours. |