| 학회 |
한국고분자학회 |
| 학술대회 |
2007년 봄 (04/12 ~ 04/13, 제주 ICC) |
| 권호 |
32권 1호 |
| 발표분야 |
나노바이오기술용 고분자(의료용 고분자 부문위원회) |
| 제목 |
Preparation and Characterization of A Novel Aptamer Biosensor Gate |
| 초록 |
A novel aptamer biosensor gate was prepared to detect the change of electronic and optical signals during and after the binding of aptamers on the chip to biomolecules. A silicon wafer pre-coated with gold (Au-Si wafer) was used as a model substrate and anti-thrombin aptamer, which specifically recognized thrombin in the blood, was used as a model aptamer with a sequence of d(GGTTGGTGTGGTTGG). A solution of thiol modified aptamers was coated on Au-Si wafer. The chip was washed and then observed with an atomic force microscope (AFM) and a scanning electron microscope (SEM), which confirmed the successful binding of anti-thrombin aptamers on the Au-Si wafer. The binding capacity of the aptamer biosensor to FITC labeled thrombin will be discussed in detail. The novel aptamer biosensor gate will be further applied for the development of aptamer-nanowire biosensors. |
| 저자 |
김기수, 한세광
|
| 소속 |
포항공과대 |
| 키워드 |
Aptamers; Thrombin; Physical Binding; Biosensors
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| E-Mail |
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