| 학회 | 한국고분자학회 |
| 학술대회 | 2004년 가을 (10/08 ~ 10/09, 경북대학교) |
| 권호 | 29권 2호, p.386 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Evaluation of bone formation with PLA/ CMP composites and bone marrow stromal cells |
| 초록 | Bioactive ceramics such as hydroxyapatite, beta-tricalcium phosphate, and calcium metaphosphate (CMP) are known to provoke specific biological responses at the interface of the materials resulting in the formation of a strong bond between the tissue and materials1-3. A new method to prepare PLA/CMP (poly-L-lactide/calcium metaphosphate) composite scaffolds was developed for effective bone tissue engineering. This Press-and-Baking method is composed of pressing the mixture of PLA, CMP, and salt particles at 150 MPa for 3 min followed by heat treatment at 210°C for 30 min. The scaffolds had a homogeneously interconnected porous structure without a skin layer, and they exhibited a narrower pore size distribution and higher mechanical strength in comparison with scaffolds made by a solvent casting method. The scaffolds were seeded by bone marrow derived osteoblasts and cultured for up to 5 weeks(Fig. 1). The number of cells attached to and proliferated on the scaffolds was in the order of ; PLA by Press-and-Baking ＜ PLA/CMP by solvent casting ＜ PLA/CMP by Press-and-Baking (Fig. 2). In addition, the alkaline phosphatase activity of and calcium deposition were enhanced significantly for the scaffolds by Press-and-Baking compared to them by solvent casting. Such a superior characteristics of the new Press-and-Baking method should be resulted from that the CMP particles could contact directly with cells/tissues to stimulate the cell proliferation and osteogenic differentiation, while the CMP particles would be coated by polymers and hindered to interact with cells/tissues in the case of a solvent casting method. Fig. 1. Bone marrow derived osteoblasts Fig. 2. Cell Growth of seeded bone marrow derived osteoblasts as function of culture condition Reference 1. Min Wang, Biomaterials, 24, 2113 (2003) 2. Cato T. Laurencin, Mohamed Attawia, Mark D. Borden, Curr Opin Orthop, 10, 445 (1999) 3. Lichun Lu, Bradford L. Currier, Michael J. Yaszemski, Curr Opin Orthop, 11, 383 (2000) |
| 저자 | 정영미1, 김상헌1, 김수현1, 김영하1, 김석영2 |
| 소속 | 1한국과학기술(연), 2영남대 |
| 키워드 | PLA; CMP; composite; bone marrow derived osteoblasts; bone tissue engineering |
