| 초록 |
In recent years, poly(DL-lactic-co-glycolic acid) (PLGA) is one of the most widely used biodegradable polymers since it is biocompatible and its degradation rate can be easily controlled by varying the copolymer ratio of lactic to glycolic acid. But this polymer as well as other common synthetic biodegradable polymers has limitations for some applications because of its hydrophobicity and thus low cell adhesiveness. In our previous study, we blended PLGA (lactic to glycolic acid molar ratio, 75:25)/Tween 80 (polysorobate 80) to improve the hydrophilicity and cell adhesiveness of PLGA. PLGA/Tween 80 blend scaffolds with different Tween 80 compositions were fabricated by modified particulate-containing thermal compression method. In this study, PLGA/Tween 80 blend scaffolds were examined for their in vitro and in vivo tissue compatibility. In vitro experiment was conducted by cell culture (chondrocyte, cell density: 5 x 106 cells/scaffold). For in vivo evaluation, the scaffolds were implanted into the cartilage of external ear and the skull of rabbits. After 4 and 8 weeks, the cartilage and bone grafts were excised. The results were evaluated by morphology and histology examination, and MTT assay.
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