| 초록 |
Micro- and nanofabrication techniques can be utilized to provide a set of mechanostimulatory signals to the cells that mimic the extracellular matrix (ECM). Here, we report periodic lateral topographic striations with an intention to mimic the ECM for investigating anticancer drug-induced cytotoxicity in stem cells. The ECM cues could facilitate perimeter, elongation, and gap junction formation of mesenchymal stem cells (MSCs), which eventually influenced the fate of cells. Interestingly, the appropriate inhibition of gap junctions of MSCs on the ECM mimicking substrates could prevent the cisplatin–induced cytotoxicity compared to that on the flat substrates. Our results imply that nanoscale topography is an important consideration, which uniquely enable the consideration and rational design of ECM-like topographic features. Furthermore, the natural topography of the ECM in the context of stem cell niches may serve as an important indicator for chemotherapeutic agent sensitivity. |
