| 학회 |
한국화학공학회 |
| 학술대회 |
2015년 봄 (04/22 ~ 04/24, 제주 ICC) |
| 권호 |
21권 1호, p.19 |
| 발표분야 |
고분자 |
| 제목 |
Stabilization of Histidine-tagged Green Fluorescent Proteins in Organic Environment Using Nitrilotriacetic acid-End-Functionalized Poly[(poly ethylene glycol) ethyl ether methacrylate] |
| 초록 |
Non-covalent bioconjugation method was used to stabilize histidine-tagged green fluorescent protein (His6-GFP). Well-defined nitrilotriacetic acid (NTA) end-functionalized Poly[poly ethylene glycol) ethyl ether methacrylate] was prepared using the initiator containing tert-butyl protected NTA moiety by atom transfer radical polymerization (ATRP). Well-defined NTA-p(PEGMA) was characterized using 1H NMR and GPC. Using end-functionalized polymers, stabilization of His6-GFP was attempted by forming micellar aggregates in solution. D-values for the stabilization of His6-GFP were determined using photoluminescent spectroscopy. Micellar aggregates were analyzed using transmission electron microscopy (TEM) and dynamic light scattering (DLS). |
| 저자 |
김봉수1, 허광선1, 하은주2, 백현종2
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| 소속 |
1경남정보대, 2부산대 |
| 키워드 |
Bioconjugation; histidine-tagged green fluorescent protein; NTA; ATRP; stabilization
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| E-Mail |
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| 원문파일 |
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