| 초록 |
In this work, our group made easy, cheap and light (2 g) portable 3D paper-based microfluidic device (PBMD). By using paper and capillary force, this device could control flow speed and direction accurately without any external device like a pump or electricity. The flow speed was controlled by the width of 3D-channel and character of the paper surface. In addition, speed control and chemical & physical barrier could control the direction of fluid flow, which moved by capillary force. Using these methods, complicated Enzyme-linked Immunosorbent Assay (ELISA) steps could be done consequently in order in the PBMD by injecting solutions (sample, washing, and enzyme’s substrate) at the same time. In 13min, all steps end and analyze color data immediately. To test this device, the selected target was thioredoxin1 (Trx1) as a breast cancer biomarker. In PBMD, it could detect range from 2 nM to 20 nM of Trx and the prepared PBMD Trx sensor displayed high sensitivity and good selectivity. |
